Böcker utgivna av Springer New York

This volume delivers a compendium of detailed protocols to the research community in order to aid in the investigation and discovery of Yersinia virulence mechanisms using important in vivo and in vitro infection models, which have led to major advances in the field and in our understanding of Yersinia pathogen-host interactions. Beginning with a section on mouse models, the book continues with chapters covering the monitoring of bacteria during infection, invertebrate models, as well as Yersinia interaction with immune cells and immune signaling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Pathogenic Yersinia: Methods and Protocols providesa single source for researchers seeking to better understand these pathogens and the diseases they produce in humans.

This book brings together recent methods and theoretical approaches developed to dissect the activity and function of bacterial and eukaryotic Structural Maintenance of Chromosome (SMC) proteins. The protocols explore numerous subjects such as depletion systems to assess SMC function, genetic manipulation of SMC proteins, chromosomal, biochemical, and microscopy-based assays of SMC activity, as well as theoretical modeling of SMC activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, SMC Complexes: Methods and Protocols will greatly aid researchers looking into the mechanism of action of SMC complexes on DNA.Chapters 10 and 19 areavailable Open Access under a CC BY 4.0 license via link.springer.com.

This detailed collection gathers both established and recent technical procedures to study the Endosomal Sorting Complex Required for Transport (ESCRT) complexes in a wide range of biological systems: Archaea, A. thaliana, U. maydis, S. cerevisiae, S. pombe, C. elegans, D. melanogaster, and mammalian cells. Opening with a section on imaging techniques, the book continues with chapters covering biochemical approaches presenting strategies for production and characterization of recombinant ESCRT proteins, or of specific ESCRT protein domains, as well as genetic and proteomic experimental approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical,The ESCRT Complexes: Methods and Protocols serves as a compact guide for researchers interested in establishing an integrated approach to investigate the ESCRT machinery functions in cell biology.

Advanced maths students have been waiting for this, the third edition of a text that deals with one of the fundamentals of their field. This book contains a systematic treatment of probability from the ground up, starting with intuitive ideas and gradually developing more sophisticated subjects, such as random walks and the Kalman-Bucy filter. Examples are discussed in detail, and there are a large number of exercises. This third edition contains new problems and exercises, new proofs, expanded material on financial mathematics, financial engineering, and mathematical statistics, and a final chapter on the history of probability theory.

This volume provides in-depth descriptions and discussion of clinical trial designs based on novel data analysis of high-quality trials for 11 different neurological conditions in order to ultimately improve the quality of care in neurology. Some of the conditions discussed in this book are stroke, traumatic brain injury, Alzheimer¿s disease, epilepsy, and tumors of the central nervous system. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your research center and clinical investigation. Thorough and comprehensive, Clinical Trials in Neurology is a valuable guide for individuals in the research, education, and clinical practice fields, specifically in the fields of medicine, physical therapy, occupational therapy, nursing, and public health. This volume is also an important resource for clinicians aiming to improve their understanding in methodology of clinical trials.

This volume covers methodologies, ranging from the molecular level to the network level, used to study receptor-receptor interactions in heteroreceptor complexes inside the central nervous system. The chapters in this book cover topics such as biochemical binding techniques; receptor autoradiography; superfused synaptosome techniques; RTK-GPCR interaction; fluorescence and bioluminiscence energy transfer methods, Co-IP cytometry-based FRET; and novel bioinformatic approaches to understand membrane heteroreceptor complexes and the global panorama of their receptor-receptor interactions. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Cutting-edge and thorough, Receptor-Receptor Interactions in the Central Nervous System is a valuable resource for any scientist or researcher interested in this field of study.

"e;The human body is a machine whose movements are directed by the soul,"e; wrote Rene Descartes in the early seventeenth century. The intr- sic mechanisms of this machine gradually became clear through the hard work of Renaissance scientists. Leonardo da Vinci is one such scientist from this period of enlightenment. In pursuit of knowledge, Leonardo dissected the bodies of more than 30 men and women. He sawed the bones lengthwise, to see their internal structure; he sawed the skull, cut through the vertebrae, and showed the spinal cord. In the process, he took extensive notes and made carefully detailed sketches. His drawings d- ferentiated muscles that run across several joints from those muscles that act on a single joint. "e;Nature has made all the muscles appertaining to the motion of the toes attached to the bone of the leg and not to that of the thigh,"e; wrote Leonardo in 1504 next to one of his sketches of the lower extremity, "e;because when the knee joint is flexed, if attached to the bone of the thigh, these muscles would be bound under the knee joint and would not be able to serve the toes. The same occurs in the hand owing to the flexion of the elbow. "e; Another Renaissance scholar who made fundamental contributions to the physiology of movement is Giovanni Alfonso Borelli. Born in 1604 in Naples, Borelli was a well-respected mathematician.

Categories for the Working Mathematician provides an array of general ideas useful in a wide variety of fields. Starting from the foundations, this book illuminates the concepts of category, functor, natural transformation, and duality. The book then turns to adjoint functors, which provide a description of universal constructions, an analysis of the representations of functors by sets of morphisms, and a means of manipulating direct and inverse limits. These categorical concepts are extensively illustrated in the remaining chapters, which include many applications of the basic existence theorem for adjoint functors. The categories of algebraic systems are constructed from certain adjoint-like data and characterized by Beck's theorem. After considering a variety of applications, the book continues with the construction and exploitation of Kan extensions. This second edition includes a number of revisions and additions, including two new chapters on topics of active interest. One is on symmetric monoidal categories and braided monoidal categories and the coherence theorems for them. The second describes 2-categories and the higher dimensional categories which have recently come into prominence. The bibliography has also been expanded to cover some of the many other recent advances concerning categories.

In its first edition, Principles of Clinical Medicine for Space Flight established itself as the authoritative reference on the contemporary knowledge base of space medicine and standards of care for space flyers. It received excellent notices and is used in the curricula of civilian and military training programs and used as a source of questions for the Aerospace Medicine Certifying Examination under the American Board of Preventive Medicine. In the intervening few years, the continuous manning of the International Space Station has both strengthened existing knowledge and uncovered new and significant phenomena related to the human in space.  The Second Edition incorporates this information. Gaps in the first edition will be addressed with the addition new and revised chapters. This edition is extensively peer reviewed and represents the most up to date knowledge.

This volume presents the latest collection of immunophenotypic techniques and applications used in research and clinical settings. Chapters in this book cover topics such as constructions of high dimensions fluorescence and mass cytometry panels; fluorescence barcoding; using dried or lyophilized reagents; and immunophenotypic examples of specific cell types. The book concludes with a discussion on the critical roles of quality control and immunophenotyping in the clinical environment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Immunophenotyping: Methods and Protocols is a valuable resource for any researchers, clinician, or scientist interested in learning more about this evolvingfield.