Böcker av Sanna Sillankorva

This highly anticipated second edition details cutting-edge experimental approaches for bacteriophage research. This comprehensive volume details new and updated experimental approaches for isolating, formulating, and engineering bacteriophage medicinal products. Authored by leading experts and written in the highly successful Methods in Molecular Biology series format, each chapter includes introductions to their respective topics, lists of the necessary materials and reagents, step-by-step reproducible laboratory protocols, and valuable tips for troubleshooting and avoiding known pitfalls.Whether you are a seasoned scientist or a newcomer, Bacteriophage Therapy: From Lab to Clinical Practice, Second Edition provides essential tools and knowledge to advance bacteriophage research and its translation into innovative medicinal products and clinical therapies.

After several years of abandonment, the use of (bacterio)phages for bacterial control has withdrawn reappraisal and several studies are ongoing on varied fields. Despite this enthusiasm, there is a lack of research on the use of phage to reduce bacteria living on surfaces in a life form known as biofilms. This work explores the potential of phages in controlling Pseudomonas fluorescens (Gram-negative) and Staphylococcus lentus (Gram-positive), two widespread inhabitants of dairy plant surfaces and products. P. fluorescens is the dominant microorganism present in the microflora of raw or pasteurized milk at the time of spoilage and frequently isolated from contaminated fresh meats and refrigerated products. S. lentus is coagulase-negative staphylococci (CoNS) and a member of the S. sciuri group which are recurrently recovered from infected bovine and caprine mammary glands. "Use of bacteriophages to control biofilms" emphasis on the isolation and thorough morphological, physico-chemical and genomic characterization of phages and furthermore focuses on efficacy studies of phages against planktonic cultures and host bacteria in mono- and dual-species biofilms.