Böcker i Methods in Molecular Biology-serien

This book focuses on recently developed High Throughput Screening (HTS) assay protocols, many involved in the ToxCast and/or Tox21 initiatives, and the relevant HTS data analysis techniques.

Early detection of cancer at the cellular level is critical. This book provides the most recent, cutting-edge methods designed to achieve the vital goals of nanotechnology-mediated early cancer detection and less toxic therapies for this devastating disease.

The kingdom Fungi constitutes an independent group equal in rank to that of plants and animals. Fungi are highly amenable to molecular work, and a few fungal species serve as model systems to study basic processes with results that are applicable to many organisms, including humans.

Plant Stress Tolerance

The study of gene regulation has been made more tractable by the design of simple expe- mental models in which a single gene can be isolated from the milieu of the organism. In - sence, the genome of the organism is dissected in such a manner that specific genes may now be introduced into an appropriate cell line .

Additional chapters detail methodologies and instrumentation for high-throughput peptide synthesis, many different applications of peptides as novel research tools and biological probes, and the design and application of fluorescent substrate-based peptides that can be used to determine the selectivity and activity of peptidases.

Neurotransmitter Methods is intended as a bench-side companion for researchers who seek to identify, locaHze, or measure neurotransmitters and/or to identify sites of neurotransmitter action.

Studies of membrane transporters have had great impact on our und- standing human diseases and the design of effective drugs. Membrane Transporters: Methods and Protocols provides various practical methodologies for the ongoing research on membrane transporters.

In Characterization of Nanoparticles Intended for Drug Delivery, expert researchers explore the latest advances in the field, providing a set of basic methods for the characterization of nanomaterials for medical use.

In this concise and systematic book, a team of experts select the most important, cutting-edge technologies used in drug delivery systems.

Leading clinicians and scientists in solid organ transplantation review the current status of the field and describe cutting-edge techniques for detecting the immune response to the allografted organ.

This Methods in Molecular Biology(TM) book covers knowledge dissemination and theoretical assessment of OR structure and function, plus development and use of expression systems and experimental functional analysis. Offers reproducible protocols, tips and more.

Chapters focus on primer design strategies for quantitative PCR, in silico PCR primer design, and primer design using software.

The huge potential for gene therapy to cure a wide range of diseases has led to high expectations and a great increase in research efforts in this area, particularly in the study of delivery via viral vectors, widely considered to be more efficient than DNA transfection.

In this book, researchers update the classic methods and introduce new approaches that go beyond basic conjugation techniques. There is emphasis on the interplay of organic synthesis, molecular biology, surface biotechnology and biomaterials engineering.

This Methods in Molecular Biology(TM) volume offers detailed protocols, with theoretical background, and step-by-step instructions on implementing synchronization, as well as current techniques for the enhanced study of regulatory mechanisms in cell cycle events.

2-D Proteome Analysis Protocols takes the researcher through the c- plete process of working with 2-D protein gels from making the protein - tract to finally identifying the proteins of interest.

Thirty-eight years after its introduction, affinity chromatography remains a key tool in the armory of separation techniques available to separation and interaction scientists.

Hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis.

Lorette Javois' timely new 2nd edition revises and updates her widely acclaimed collection of step-by-step immunocytochemical methods, one that is now used in many biological and biomedical research programs.

Recent advances in genetics over the last quarter of a century, especially in molecular techniques, have dramatically reduced the cost of determining genetic markers and hence opened up a field of research that is increasingly helping to detect, prevent and/or cure many diseases that afflict humans.

With significant developments in the areas of chromatography and spectroscopy as well as the unique inherent chemical diversity of natural products, vital in drug research, natural products research has gained new momentum.

The basic principle of electron crystallography is to calculate a 3D density map by combining the amplitudes obtained from electron diffraction patterns with the experimental phases calculated from images of two-dimensional crystals of membrane or soluble proteins.

This Methods in Molecular Biology(TM) book offers core techniques and approaches commonly used in studying rice molecular biology and functional genomics. Includes protocols, materials lists, troubleshooting tips and advice on avoiding common pitfalls.

Dopamine, a catecholamine transmitter, plays a number of vital physiological roles in the brain and body, and, in recent years, studies on the role of dopamine in disease have opened new avenues of research and discovery.

This Methods in Molecular Biology book offers in vitro and in vivo protocols on mutation assays, cytogenetic techniques, primary DNA damage, alternate assays to animal models and updated ICH guidelines. Includes material lists, troubleshooting tips and more.

The existence of genes for RNA molecules not coding for proteins (ncRNAs) has been recognized since the 1950's, but until recently, aside from the critically important ribosomal and transfer RNA genes, most focus has been on protein coding genes.

In Cereal Genomics: Methods and Protocols, expert researchers provides modern protocols for the analysis and manipulation of cereal genomes.

The second edition of Bone Marrow and Stem Cell Transplantation expands upon the previous edition with current, detailed methods on HLA, minor-HLA and Killer Immunoglobulin Like Receptor typing.