Böcker i Methods in Molecular Biology-serien

Chapters focus on of innate immune receptors, "atypical"inflammasomes, biochemical and novel bioluminescencetechniques for the measurement of IL-1b,bioluminescent probe, biochemical and microscopytechniques, techniques to measure caspase-1 activation, cellfree systems for the study of inflammasome function, and inflammasomeactivation.

This volume provides acollection of protocols and approaches for the creation of novel ligand bindingproteins, compiled and described by many of today's leaders in the field ofprotein engineering.

This volume details several important databases and data mining tools. Data Mining Techniques for the Life Sciences, Second Edition guides readers through archives of macromolecular three-dimensional structures, databases of protein-protein interactions, thermodynamics information on protein and mutant stability, ¿Kbdock¿ protein domain structure database, PDB_REDO databank, erroneous sequences, substitution matrices, tools to align RNA sequences, interesting procedures for kinase family/subfamily classifications, new tools to predict protein crystallizability, metabolomics data, drug-target interaction predictions, and a recipe for protein-sequence-based function prediction and its implementation in the latest version of the ANNOTATOR software suite. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Data Mining Techniques for the Life Sciences, Second Edition aims to ensure successful results in the further study of this vital field.

This volume containsprotocols specifically designed for studying programmed cell death, and alsodiscusses recent advances in techniques that span broader areas of biology thathave been recently used or that have potential to be incorporated into cell deathresearch.

With numerous methods available for the quantification ofnitric oxide (NO), this detailed book explores their advantages anddisadvantages in order to provide a foundation for further research in plantnitric oxide.

This volume provides a comprehensive collection of classical and cutting edge protocols and techniques to examine the normal development and physiological functions of the gastrointestinal system and to model the most common digestive diseases.

While additional chapters provide methods toculture human and mouse dendritic cells, protocols for the isolation ofdendritic cells, the isolation of dendritic cell progenitors from mouse, andthe purification of dendritic cells from human blood.

This detailed volume explores in silico methods forpharmaceutical toxicity by combiningthe theoretical advanced research with the practical application of the tools.Beginning with a section covering sophisticated models addressing the bindingto receptors, pharmacokinetics and adsorption, metabolism, distribution, andexcretion, the book continues with chapters delving into models for specifictoxicological and ecotoxicological endpoints, as well as broad views of themain initiatives and new perspectives which will very likely improve our way ofmodelling pharmaceuticals. Written for the highly successful Methods in Molecular Biology series,chapters include the kind of detailed implementation advice that is key forachieving successful research results.Authoritative and practical, In Silico Methods for Predicting DrugToxicity offers the advantage of incorporating data and knowledge fromdifferent fields, such as chemistry, biology, -omics, and pharmacology, toachieve goals in this vital area of research.

Thissecond edition expands upon the previous volume with new and updated chapters. Auditory and Vestibular Research: Methodsand Protocols, Second Edition guides readers through protocols on cellculture, tissue engineering, nanotechnology, high-throughput screening, andphysiology. Chapters on physiology covertechniques that include optical coherence tomography, patch clamping, andphotostimulation of caged neurotransmitters. Written in the highly successful Methods in Molecular Biology seriesformat, chapters include introductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.Authoritativeand cutting-edge, Auditoryand Vestibular Research: Methods and Protocols, Second Edition aims to ensure successfulresults in the further study of this vital field.

Thisvolume covers multidisciplinary approaches on various aspects of ChikungunyaVirus (CHIKV) research that was brought together from leading laboratoriesacross the globe. Topics covered include techniques in clinical and diagnosticvirology, basic protocols in cell and virus culture, bioinformatics, andproteomics approaches in cellular response studies. Chikungunya Virus:Methods and Protocols also discusses methods in immunology and animal modelstudies, as well as different strategies of antivirals and vaccine developmentfor therapeutics against CHIKV infection. Written in the highly successful Methodsin Molecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary material and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting and avoidingknown pitfalls.Thorough and practical, Chikungunya Virus: Methods and Protocols is auseful and comprehensive resource for CHIKV researchers and anyone interestedin learning more about the Chikungunya Virus.

This volumepresents detailed laboratory protocols for invitro synthesis of mRNA with favorable properties, its introduction intocells by a variety of techniques, and the measurement of physiological andclinical consequences such as protein replacement and cancer immunotherapy.

Inaddition to updating important core techniques this third edition presents newchapters on assessing leukocyte involvement in angiogenesis, lymphatic cell andpericyte isolation techniques, spheroid and arterial ring based in vitroassays, and on pericyte involvement in angiogenesis.

This volume details a valuable collection of protocols andreviews, such as emerging experimental and theoretical approaches. Theseapproaches have resulted in a substantial improvement in the understanding ofchromosome architecture. Chromosome Architecture:Methods and Protocols guides readers through cutting-edge interdisciplinarymethods which allow for an understanding of architecture of chromosomes withexceptionally enhanced resolution, both in terms of space and time. Written in the highly successful Methods inMolecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.Authoritativeand cutting-edge, ChromosomeArchitecture: Methods and Protocols aims to ensure successful results in thefurther study of this vital field.

This detailed volume encompasses chapters from leadingexperts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functionalproduction and stabilization of recombinant integral membrane proteins (IMPs).

The aim of this volume is to present current methodologies to predict and understand the pathogenesis of Type-1 Diabetes for clinical and non-clinical researchers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and reliable, Type-1 Diabetes: Methods and Protocols will aid researchers in using these methods to advance their own studies.

Presenting topics from the basic application of molecular genetics to more complex gene expression analysis using different models of study, this detailed volume explores asthma through the lens of genetics, considered to play an essential role in the etiopathogenesis of the disease.

Herein, these models are appliedto a wide field of disease areas, including diabetes, cardiovascular disease,skin disorders, cancer, neurodegenerative and neuromuscular diseases, retinaldisorders, as well as various behavioral models.

Presentingstate-of-the-art protocols to study gap junctions, this detailed book firstfocuses on the use of methods and tools to investigate the different aspects ofconnexin expression and gap junction regulation.

Chapters in the book cover many facets ofWNV, such as propagation and titration of WNV in vero cells, examination of WNVneuroinvasion and neuropathogenesis in the central nervous system of a murinemodel, field surveillance methods for WNV, and in vitro and in vivoblood-brain barrier models to study WNV pathogenesis.

This third edition volumeexpands on the previous editions both by presenting more detailed protocols forthe techniques described in the first and second editions of High ThroughputScreening: Methods and Protocolsand by covering important new procedures. The first chapter ofthis book provides an overview of important assay development techniques, whilethe rest of the chapters detail how to develop and execute screens at whateverthroughput the user needs. Some chapter examples are: structure-based virtualscreening, high throughput screening using mass spectrometry, identification of state-dependentblockers for voltage gated calcium channels, bioluminescence resonanceenergy transfer platform to monitor protein-protein interactions in live cells,high throughput flow cytometry, and application of imaging-based assays in microplate formats for highcontent screening. Written in the highly successful Methods in MolecularBiology series format, chapters include introductions to their respectivetopics, lists of the necessary materials and reagents, step-by-step, readilyreproducible laboratory protocols, and tips on troubleshooting and avoidingknown pitfalls.Cutting edge and thorough, High Throughput Screening: Methods and Protocols,Third Edition, is a valuable resource for anyone who is interested in HTSresearch.

This volume bringstogether the most widely used and important protocols currently being employedin researching and understanding bacterial cell wall homeostasis. Chapters in BacterialCell Wall Homeostasis cover a variety of subjects, such as: modernmicroscopy techniques and other biophysical methods used to characterize thesubcellular structure of the bacterial cell wall; high-throughput approachesthat can be used to identify all the genes and proteins that participate in thecorrect functioning of an organism¿s cell wall; protocols for assayingindividual gene products for specific cell wall functions or identify chemicalswith inhibitory activity against the cell wall; and methods for analyzing the non-proteincomponents of the cell wall and the increasing use of computational approachesfor predicting and modeling cell wall related functions and processes. Writtenin the highly successful Methods in Molecular Biology series format,chapters include introduction to their respective topics, lists of thenecessary material and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.Thoroughand cutting-edge, Bacterial Cell Wall Homeostasis: Methods and Protocolsemphasizes the diversity of the research taking place in bacterial cell wallhomeostasis, and explains how the integration of information from acrossmultiple disciplines is going to be essential if a holistic understanding ofthis important process is to be obtained.

This volume contains collection of Natural Killer Cell methodologies relevant for both basic and translational research. These methodologies present new developments in the natural killer (NK) cell field, such as understanding the influence of NK cells metabolism on its function, identifying complexity of NK cell subsets through mass cytometry, and determining the emergence of memory NK cells in murine model of MCMV infection. Methods that study NK cell migration and cytotoxicity through endpoint analysis or live single cell imaging are also discussed. Chapters also describe methods pertaining to translational application of NK cells, such as ex vivo expansion of NK cells on K562 cell lines genetically modified to express either membrane bound IL-15 or membrane bound IL-21, large scale NK cell culture, current techniques for engineering NK cells to express chimeric antigen receptors or chemokine receptors using retroviral vectors, electroporation of mRNA, and the natural phenomenon of trogocytosis. Written in the highly successful Methods in Molecular Biology series format, these chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting edge and thorough, Natural Killer Cells: Methods and Protocols is a valuable resource for researchers who not only want to understand mechanisms that govern NK cell behavior and diversity, but also for those who want to understand how to systematically evaluate NK cells for adoptive immunotherapy applications.

This volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field ofnon-viral gene delivery vectors.

Authoritative and practical, In Vivo Fluorescence Imaging: Methods and Protocols serves as a valuable reference for researchers from numerousfields who wish to become more familiar with in vivo fluorescence imagingtechniques.

The NuclearReceptor Superfamily, Second Edition will benefit those starting out in thenuclear receptor research field as well as to more established researchers whowish to apply different methods to a particular receptor or research problem.

This volume provides current methods and protocols for geneand protein delivery based on both lentivirus-generated and spontaneouslyreleased nanovesicles.

Plant Proteostasis: Methods and Protocols is broken into four parts focusing on thestudy of ubiquitin-dependent post-translational modifications, protocolsfocused on Ubl post-translational modifications, protein homeostasis, andprotocols for the in silico analysis.

This volume presents the latest protocols for both laboratory andbioinformatics based analyses in the field of marine genomics. The chapterspresented in the book cover a wide range of topics, including the sampling andgenomics of bacterial communities, DNA extraction in marine organisms,high-throughput sequencing of whole mitochondrial genomes, phylogenomics, SNPdiscovery, SNP-arrays for species identification, digital PCR-basedquantification methods, environment DNA for invasive species surveillance andmonitoring, microarrays for the detection of waterborne pathogens, DNAbarcoding of marine biodiversity, metabarcoding protocols for marineeukaryotes, analytical protocols for the visualization of eukaryotic diversity,and applications for genomic data to benthic indices for environmentalmonitoring. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introduction to their respective topics, listsof the necessary materials and reagents, step-by-step, readily reproduciblelaboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Marine Genomics: Methods and Protocols is avaluable resource for researchers, students, and policy makers in the field ofmarine biology.

This volume discusses methods for the study of multipotent and pluripotentstem cells of the hair follicle. The stem cells described are involved in boththe growth of the hair follicle and its production of the hair shaft, as wellas the growth of the hair follicle sensory nerve. Multipotent Stem Cells ofthe Hair Follicle: Methods and Protocols also explores very unexpectedresults such as that of the hair follicle-associated-pluripotent (HAP) stemcells, which not only have the capability for regenerating the hair folliclesensory nerve, but also can differentiate ex vivo and in vivo to multiple celltypes not associated with the hair follicle¿these include glial cells, motorneurons, and beating cardiac muscle cells. The potential for HAP stem cells forregenerative medicine is also discussed in detail. Written in the highly successful Methods inMolecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.Cutting-edgeand thorough, Multipotent Stem Cells of the Hair Follicle: Methods andProtocols is a valuable resource for researchers interested in thisfield.

This volumepresents detailed laboratory protocols regarding the three major routetechnologies luciferases, efficient opticalprobes, and applications to visualizing molecular events in living subjects.