Böcker i Methods in Molecular Biology-serien

This detailed book explores strategies that have been developed to combat plant virus infection. Beginning with a section on techniques for identifying and studying the virus resistance gene involved in plant innate immunity, the volume continues by delving into techniques related to novel mechanisms of plant virus resistance, methods for the analysis and practical use of RNA silencing, as well as methods for the development of plant viral vaccines. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Antiviral Resistance in Plants: Methods and Protocols serves as an ideal guide for researchers working to combat the serious threats plant virus diseases represent for agricultural production and beyond.

Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting and avoiding known pitfalls.

This volume introduces different concepts and methods of detecting RNA in biological material in a variety of model systems.

This volume covers a wide range of various fields of research, with the common thread being Next Generation Sequencing (NGS) related methods and applications, as well as analysis and interpretation of the data obtained. Chapters guide readers through the highly dynamic processes of translational and transcriptional profiling of a cell, method to detect copy number alterations (CNAs), targeted sequencing applications,  method called “Hi-Plex” to characterize known polymorphic loci, single-cell of DNA or RNA, identify and characterize rare circulating CD4 T cells, and computational pipeline for RNAseq analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Next Generation Sequencing: Methods and Protocols aims to be useful and informative for further study into this vital field. 

The volume presents a variety of protocols that will be useful to researchers working in the field of disease modeling, drug discovery, and the cell biology of various tissues. Chapters in this book cover topics such as requirements and preconditions for using human induced pluripotent cell lines in assay development; the culture of iPSCs and iPSC-derived cardiomyocytes by bioreactor technologies; distinctions between hepatocytes, vascular endothelial cells, cardiomyocytes, renal podocytes, and neurons; and high content assays of mitochondrial function and autophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cell-Based Assay using iPSCs for Drug Development and Testing: Methods and Protocols is a valuable resource for researchers working in this important and developing field of study.

The volume explores methods for isolation and characterization of lipids, and their production using various oleaginous organisms. The protocols presented in the book consider sugars derived from different substrates including chemically pre-treated agricultural residues, industrial residues containing lignin, food wastes, and industrial waste water in an approachable format. The book also discusses applications that use oleaginous organisms to transform substrates into a variety of products including bio-crude, high value fatty acids, biofuels such as biodiesel, neutral lipids, volatile fatty acids, and surfactants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Microbial Lipid Production: Methods and Protocols is a valuable resource aimed at novice researchers who are working on improving their knowledge and skills in this developing field.

The volume brings together some of the best experts in the field of modern metabolomics to discuss various techniques used today to study specific metabolite classes, and metabolomics in bacterial systems and mammalian systems.

This detailed book addresses the continual development and application of new methods and protocols to understand the biology of the pathogen Neisseria gonorrhoeae. Beginning with gonococcus biology and antimicrobial resistance, the volume continues with areas of study such as producing major gonococcal antigens to high purity, genetic transformation of gonococci and strategies for global RNA sequencing, evaluation of potential vaccine/drug targets and the assessment of hypothetical protein function, as well as a number of methods and protocols for studying the biological interactions of the gonococcus and the host. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Neisseria gonorrhoeae: Methods and Protocols provides researchers with a foundation for progress toward the eventual control of this disease and a significant reduction in global case numbers of gonorrhea.

The volume outlines techniques used to study the primary mechanisms of important DNA repair pathways.

This book encompasses broad aspects of Cryptosporidium research with established methods that have been improved and expanded over the years as well as recent cutting-edge techniques. Within this collection are numerous molecular methods as well as protocols for genotyping and diagnostics, while also including room for in vitro cell culture techniques to address the issues with growing this difficult organism continuously. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cryptosporidium: Methods and Protocols serves as an idea guide to the inherent challenges of working with cryptosporidial parasites to provide a foundation for new investigators to build upon.The chapter "Accessing Cryptosporidium Omic and Isolate Data via CryptoDB.org" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

This book expands upon the useful first edition by exploring classic Quantitative Polymerase Chain Reaction (qPCR) techniques as well as a number of recently developed applications.

This book provides key methods, approaches, and strategies to dissect the plant defense response.

This detailed volume examines bioinformatic and molecular biological methods useful to identify and to explore the functions of CpG islands, key navigation points to understand gene regulation in fundamental processes such as development and cell differentiation as well as in diseases like cancer.

DNA Replication

This book presents a selection of current capillary electrophoresis methods used to separate representative types of molecules and particles and in combination with different detection techniques.

This volume includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, sulfur-containing RNAs, viral and satellite RNAs, RNA isoforms, and alternatively spliced RNA variants from various organisms, as well as key discussions of computational data processing for genome-wide datasets.

Stem cell mobilization represents a transient increase in the levels of circulating stem and progenitor cells.

Cell migration is a key component of many biological processes. This book provides a catalogue of techniques for the study of cell migration. It brings together a wide range of these techniques from the basic migration assays to recent technical advances.

This book describes the methodologies and approaches in systems metabolic engineering and provides a step-by-step guide for their implementation. Includes readily reproducible laboratory protocols and tips on troubleshooting.

Imaging and Tracking Stem Cells: Methods and Protocols gathers representative protocols related to the vital techniques of stem cell imaging and lineage tracing, including that of live cells, both in vivo and in vitro.

Stem Cell Nanotechnology: Methods and Protocols gathers several representative protocols related to the emerging interest in nanotechnology as it relates to stem cell biology.

Biomass conversion research is a combination of basic science, applied science, and engineering testing and analysis. In Biomass Conversion: Methods and Protocols, expert researchers in the field detail methods which are now commonly used to study biomass conversion.