Böcker i Methods in Molecular Biology-serien

This changing de?nition of disease means that today's undergraduate or graduate student in dentistry (and its related ?elds) must be in a position not only to acquire new knowledge in the future but also to be able to evaluate the information and apply it in a clinically relevant setting.

The kingdom Fungi constitutes an independent group equal in rank to that of plants and animals. Fungi are highly amenable to molecular work, and a few fungal species serve as model systems to study basic processes with results that are applicable to many organisms, including humans.

Recent efforts to characterize genetic variation in the human genome, coupled with the rapidly developing field of genomics, have lead directly to the development of new and innovative approaches to the identification of genes contributing to complex human diseases.

This volume covers an array of topics from the employment of the chick CAM model using fluorescent proteins and other fluorescent probes, to intravital fluorescent imaging, as well as 3-dimensional imaging, and design instructions on how to create new and improved far-red and infrared fluorescent proteins, to name a few.

Chapters outline methods to study miRNA functions in various cell types from a single cell type level to entire model organisms, and present studies of miRNAs in the context of viruses and the immune response.

Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required.

Chemical library technologies have brought about dramatic changes in the drug discovery process, and, though still evolving, they have become an integral part of ongoing drug discovery research.

Driven by methodological success in identifying reliable lineage markers, regulatory T cells have quickly been recognized as the most numerous subset of immune regulators in the body with critical functions in a wide array of immune responses.

Vertebrate Embryogenesis: Embryological, Cellular and Genetic Methods attempts to address the increasingly important need of straddling species boundaries in the context of a single research program by compiling research protocols used in a wide range of vertebrate species.

Due to their novel concepts and extraordinary high-throughput sequencing capacity, the "next generation sequencing" methods allow scientists to grasp system-wide landscapes of the complex molecular events taking place in various biological systems, including microorganisms and microbial communities.

Now we can accurately predict cis-regulatory modules, conserved cl- ters of binding sites (Chapters 13 and 15), partly based on high-throughput ch- matin immunoprecipitation experiments in which tens of millions of DNA segments are sequenced by massively parallel, next-generation sequencers (ChIP-seq, Chapters 9, 10, and 11).

The proteome consists of a complex mixture of proteins each of which need to be folded correctly in order to function for the health of the organism, and many of these proteins require molecular chaperones to reach the correct conformation and, in some cases, to remain in a folded form.

Written in the successful Methods in Molecular Biology (TM) series format, In Vivo NMR Imaging: Methods and Protocols aims to be an experimental compendium of modern in vivo MR imaging with special focus on recent developments in molecular imaging and new protocols for imaging metabolism and molecular markers.

In The Plant Cell Wall: Methods and Protocols, experts in the field describe detailed methods which are currently being applied to investigate the many aspects of the plant cell wall including its structure, biochemical composition, and metabolism.

Cell cycle checkpoints control the fidelity and orderly progression of eukaryotic cell division. By controlling the orderly progression of critical cell cycle events such as DNA replication and chromosome segregation and ensuring proper repair of damaged DNA, cell cycle checkpoints function to ensure genome integrity. Mechanisms of checkpoint controls are not only the research focus of investigators interested in mechanisms that regulate the cell cycle, but are also the interests of researchers studying cancer development as it is increasingly clear that loss of cell cycle checkpoints, which leads to genomic instability as a result, is a hallmark of tumorigenesis. Cell Cycle Checkpoints: Methods and Protocols provides detailed descriptions of methodologies currently employed by researchers in the field, including those commonly used in the mammalian, yeast, C. elegans, Drosophila, and Xenopus model systems. Each chapter describes a specific technique or protocol, such as a method to induce cell cycle checkpoints in a particular model system, to synchronize a population of cells to allow observations of cell cycle progression, to identify genes involved in checkpoint regulation, and to study particular protein components of cell cycle checkpoint pathways. Written in the highly successful Methods in Molecular Biology¿ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Cell Cycle Checkpoints: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this essentialfield.

Characterization of copy number variation and other forms of structural genetic variation has highlighted the complexity of human genetic variation and also provided significant insight into the evolution and dynamic nature of our genome.

The last fifteen years have witnessed the birth and maturation of many original methods and the development of protocols specific to single molecule measurements and their analysis, including techniques involving optical imaging, electron microscopy, optical and magnetic trapping, and developments in atomic force microscopy.